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Updated: Jan 9, 2026

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Molecular-scale isotropic 3D super-resolution microscopy via interference localization.

Shihang Luo1, Xian'ao Zhao1,2, Yuanyuan Li1

  • 1Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

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|December 2, 2025
PubMed
Summary
This summary is machine-generated.

ROSE-3D is a new interferometric localization method for isotropic 3D super-resolution microscopy. It significantly enhances imaging precision, enabling detailed visualization of subcellular structures like nuclear lamins and DRP1 protein assemblies.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • 3D nanoscale imaging is crucial for understanding subcellular structures.
  • Conventional single-molecule localization microscopy has limited axial resolution, hindering detailed 3D visualization.
  • Astigmatism-based methods offer limited depth of field and precision.

Purpose of the Study:

  • To introduce ROSE-3D, an interferometric localization approach for isotropic 3D super-resolution imaging.
  • To improve both lateral and axial localization precision across a substantial depth of field.
  • To enable multicolor and whole-cell imaging for in situ nanoscale organization studies.

Main Methods:

  • Development of ROSE-3D, an interferometric localization technique.
  • Characterization of imaging performance, including lateral and axial precision over depth of field.
  • Application of ROSE-3D for multicolor and whole-cell imaging of cellular structures.

Main Results:

  • ROSE-3D achieves isotropic 3D super-resolution imaging with uniform performance.
  • It improves lateral localization precision by 2-6 times and axial precision by 3.5-8 times compared to astigmatism-based methods.
  • ROSE-3D successfully resolved the nanoscale organization of nuclear lamins and DRP1 protein assemblies in situ.

Conclusions:

  • ROSE-3D significantly enhances 3D super-resolution imaging capabilities.
  • This technique provides unprecedented precision for visualizing nanoscale cellular architecture.
  • ROSE-3D is a powerful new tool for interrogating complex cellular structures in their native environment.