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Updated: Jan 9, 2026

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
Published on: June 16, 2023
LncRNA FEZF1-AS1 regulates the gene expression and alternative splicing associated with osteosarcoma
Yanlong Han1, Ziang Ye1, Li Wang2
1Department of Bone and Joint Surgery Center, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China.
Background:
Osteosarcoma (OS) faces a high metastatic burden, limited effective treatments, and a lack of specific therapeutic targets.
Methods:
In this prospective study, we established a human osteosarcoma cell (HOS) model overexpressing lncRNA FEZF1-AS1 (OE FEZF1-AS1), and subsequently obtained FEZF1-AS1-affected transcriptome data using high-throughput RNA sequencing (RNA-seq).
Results:
OE FEZF1-AS1 promoted HOS cell proliferation and invasion, upregulated 125 genes, suppressed 48 genes, and impacted 1156 alternative splicing events (ASEs), including pathways like C-type lectin receptor signaling and Yersinia pestis infection.
Conclusions:
LncRNA FEZF1-AS1 plays a pivotal role in promoting the proliferation and invasion of HOS cells. Moreover, our study provides a comprehensive gene screening approach for identifying potential targets of FEZF1-AS1, emphasizing their regulatory mechanisms and ASEs in OS.
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