Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Lingo01:11

Genetic Lingo

113.6K
Overview
113.6K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.1K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.1K
What is Genetic Engineering?00:49

What is Genetic Engineering?

79.6K
Overview
79.6K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

16.0K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
16.0K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.6K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.6K
The Central Dogma01:25

The Central Dogma

138.8K
Overview
138.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visible light reprograms MSCs and T cells into tumor-suppressive states via OPN4-mediated epigenetic remodeling.

Acta pharmacologica Sinica·2026
Same author

Activation-induced SLC7A1 expression enhances T cell sensitivity to cGAMP-mediated STING signaling.

Cell reports·2026
Same author

Structure-Guided Discovery of Selective Polo-Like Kinase 3 Inhibitors.

ACS medicinal chemistry letters·2026
Same author

"We don't know what we don't know": rural council officers' perceptions of the gendered impacts of climate change in Australia.

BMC public health·2026
Same author

Clonal lineage tracing of innate immune cells in human cancer.

Cancer cell·2026
Same author

microRNA-25 drives immune checkpoint therapy resistance by repressing innate and humoral immunity via Syndecan-3.

Nature communications·2026

Related Experiment Video

Updated: Jan 9, 2026

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.7K

A unified genetic perturbation language for human cellular programming.

Austin Hartman1,2,3, Oliver Takacsi-Nagy1,4,3, Courtney Kernick1

  • 1Department of Pathology, Stanford University, Stanford, CA, USA.

Biorxiv : the Preprint Server for Biology
|December 3, 2025
PubMed
Summary
This summary is machine-generated.

CRISPR-All unifies genetic perturbation technologies for simultaneous, combined genome-scale engineering in human cells. This breakthrough enables comprehensive analysis and optimization of cellular functions for biological and clinical applications.

More Related Videos

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
09:37

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Published on: March 5, 2017

13.5K
Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

1.9K

Related Experiment Videos

Last Updated: Jan 9, 2026

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.7K
Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
09:37

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Published on: March 5, 2017

13.5K
Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

1.9K

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Genomics

Background:

  • Genetic perturbations (knockouts, knockdowns, overexpression) are crucial for understanding cell function.
  • Existing perturbation technologies are largely incompatible, limiting combinatorial approaches.
  • Need for a unified system to explore complex genetic interactions at scale.

Purpose of the Study:

  • To develop a unified genetic perturbation language, CRISPR-All, for programming diverse genetic modifications.
  • To enable simultaneous and combinatorial genetic perturbations at genome scale in primary human cells.
  • To facilitate functional comparisons and screening of genetic enhancements, particularly in CAR-T cells.

Main Methods:

  • Developed a standardized molecular architecture for major perturbation classes.
  • Created a functional syntax for combining elements across perturbation classes.
  • Integrated single-cell compatible barcodes and a DNA sequence generation tool for high-level programming.

Main Results:

  • CRISPR-All successfully enabled simultaneous, combinatorial genetic perturbations in human cells.
  • Enabled head-to-head functional comparisons of perturbation types in CAR-T cell enhancement.
  • Revealed diverse phenotypic states and additive functional improvements through multiplexed perturbations.

Conclusions:

  • CRISPR-All provides a unified language for programming complex genetic changes.
  • Enables exploration of combinatorial genetic perturbation space for biological discovery.
  • Has significant potential for advancing cell engineering in biological research and clinical applications.