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Updated: Jan 9, 2026
Safety Precautions and Operating Procedures in an ABSL-4 Laboratory: 1. Biosafety Level 4 Suit Laboratory Suite Entry and Exit Procedures
Published on: October 3, 2016
[Laboratory Biosafety Guideline (2025) Revision]
Ji Yeong Lee1, Kyung-Hwan Oh1, Jeonghwa Shin1
1질병관리청 진단분석국 생물안전평가과.
Objectives:
The Korea Disease Control and Prevention Agency sought to revise the Laboratory Biosafety Guideline, creating a fourth (2025) edition of the Laboratory Biosafety Guideline, to provide updated safe laboratory environment for researchers at domestic medical and bioscience research institutions.
Methods:
Domestic and international biosafety-related laws, regulations, and criteria were investigated and analyzed.
Results:
The fourth edition of the Laboratory Biosafety Guideline (2025) was found to provide guidance on international laboratory-biosafety criteria, such as risk-assessment procedures, accident-prevention policies, and response measures, that corresponded to the World Health Organization Laboratory Biosafety Manual, 4th edition and the Biosafety in Microbiological and Biomedical Laboratories, 6th edition of the U.S. Centers for Disease Control and Prevention.
Conclusions:
The revised Laboratory Biosafety Guideline (2025) are expected to provide a safe experimental environment for researchers, with guidelines for preventing both laboratory infections and infectious-material leaks in research facilities handling pathogens and exposure to pathogens in the community. The guidelines will serve as an excellent reference for researchers managing laboratory biosafety.
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