Study on the analgesic mechanism of massage on NPP by LNCRNAH19 regulating miR-342-3p/IER3

Using a rat model of neuropathic pain, this study investigated the analgesic mechanism of massage therapy with an emphasis on the lncRNA H19/miR-342-3p/IER3 signaling pathway. Rats were split into control, model, sham, massage and lentiviral intervention groups using a spinal nerve ligation (SNL) paradigm. Molecular tests (qRT-PCR, Western blotting), behavioral evaluations (paw withdrawal thresholds and latencies) and ELISA were carried out. Massage reduced mechanical and thermal hyperalgesia by dramatically upregulating LncRNA H19 and IER3 expression and downregulating miR-342-3p and IL-17 levels. The effects of massage were replicated by intrathecal delivery of agolnc-H19a, thereby confirming H19's role in modulating the miR-342-3p/IER3 axis. By focusing on inflammatory signaling via the lncRNA H19/miR-342-3p/IER3 pathway, these results imply that massage alleviates neuropathic pain, providing new molecular understanding of the therapeutic benefits of massage.