Parallel Impedivity Measurements for Enzyme Activity Quantification in Microtiter Plates for Calcite Precipitation...

Conductometry is a widely used technique in enzyme studies as it allows researchers to monitor the activity of enzymes in solution. The determination of enzymatic activity, a time-dependent parameter, inherently takes several minutes, making it time-consuming to measure multiple samples with traditional methods. To overcome these limitations, we present a device for efficient, parallel, impedimetric measurement of urease activity. In this study, urease is representative of all enzymes that catalyze hydrolysis reactions leading to ion formation. The results show the general applicability of the device for parallelized measurement of urease activity. Further optimization is required to ensure accuracy, reliability, and reusability of the measurement set-up. Overall, at this point of development, the 24-well microplate setup is a promising tool for fast and easy enzyme activity determination, with potential applications in enzyme-based diagnostics development or geotechnical engineering and research.