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Related Experiment Videos

Cell-cycle analysis in 20 minutes.

H A Crissman, R A Tobey

    Science (New York, N.Y.)
    |June 21, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Mithramycin binds to DNA in fixed mammalian cells, fluorescing with cellular DNA content. This enables rapid cell-cycle analysis and continuous kinetic monitoring using flow cytometry.

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    Area of Science:

    • Cell Biology
    • Biophysics
    • Biochemistry

    Background:

    • Cell cycle analysis is crucial for understanding cell proliferation and development.
    • Accurate DNA content measurement is fundamental to cell cycle studies.

    Purpose of the Study:

    • To introduce a novel method for quantitative DNA fluorescence measurement in mammalian cells.
    • To enable rapid and continuous monitoring of cell cycle kinetics.

    Main Methods:

    • Mammalian cells were fixed in aqueous ethanol.
    • Mithramycin was used as a DNA-binding fluorescent dye.
    • Quantitative fluorescence was measured using a high-speed flow system instrument.

    Main Results:

    • Mithramycin fluorescence intensity is directly proportional to cellular DNA content.

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  • The flow system instrument provides rapid cell-cycle analysis.
  • Continuous monitoring of cell-cycle kinetics during experiments is now feasible.

    • Conclusions:

    • Mithramycin-based quantitative fluorescence offers a powerful tool for cell cycle analysis.
    • This method allows for unprecedented real-time kinetic studies of cell populations.