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Related Experiment Video

Updated: Jul 19, 2026

Quantitation of Rabies Virus in Various Bovine Brain Structures

Quantitation of Rabies Virus in Various Bovine Brain Structures

Published on: May 22, 2021

One-step approach producing barcoded rabies virus with optimized diversity.

Kang Tan¹, Zi-Xuan Shen², Ya-Qian Wang¹

¹Lingang Laboratory, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, 555 Qiangye Road, Shanghai 201210, China.

Cell Reports Methods

|December 3, 2025

Summary

Researchers developed a simplified one-step method for producing barcoded rabies virus strains, improving barcode diversity and efficiency for brain-wide neuronal connectivity mapping.

Keywords:

CP: neuroscience CVS-N2c strain barcoded rabies virus lateral hypothalamic area monosynaptic tracing one-step approach rabies virus production

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Published on: August 18, 2023

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Mapping neuronal connectivity is crucial for understanding brain function.
Barcoded rabies viruses are powerful tools for connectomics, but face challenges in barcode diversity and transsynaptic transfer.
Existing methods for producing barcoded CVS-N2cΔG strains are technically demanding.

Purpose of the Study:

To introduce a simplified one-step method for producing SAD-B19ΔG and CVS-N2cΔG rabies virus strains.
To improve barcode diversity and uniformity in rabies virus libraries.
To enhance the efficiency and accessibility of barcoded rabies virus production for connectomic studies.

Main Methods:

Developed a streamlined one-step protocol for generating barcoded SAD-B19ΔG and CVS-N2cΔG rabies virus strains.
Focused on simplifying the production process, reducing time, and minimizing background contamination.
Evaluated the diversity, uniformity, and tracing efficiency of viruses produced using the new method.

Main Results:

The one-step method significantly simplifies production and reduces manufacturing time.
Achieved improved diversity and uniformity of the rabies virus barcode library.
Demonstrated that viruses produced by this method exhibit tracing efficiency comparable to conventional techniques.
Eliminated background contamination issues associated with previous methods.

Conclusions:

The novel one-step method offers a more efficient and accessible approach to producing barcoded rabies viruses.
This advancement facilitates the development and application of barcoded-rabies-virus-based connectomic studies.
The improved barcode diversity and transfer efficiency will aid in comprehensive brain-wide neuronal mapping.