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MAPK pathway components AoSho1 and AoOpy2 regulate growth, sporulation, osmoregulation, and pathogenicity in

Xiaoli Li1, Lirong Zhu1, Guiqiu Luo1

  • 1State Key Laboratory for Conservation and Utilisation of Bio-Resources in Yunnan, Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, and School of Life Science, Yunnan University, Kunming, 650032, China.

Fungal Biology
|December 3, 2025
PubMed

Insights

Sho1 (Sln1-Hog pathway) and Opy2 (osmosensing pathway) are crucial for nematode-trapping fungus Arthrobotrys oligospora. Their absence impairs growth, spore production, and nematode capture, highlighting their roles in fungal pathogenicity.

Area of Science:

  • Mycology
  • Molecular Biology
  • Fungal Pathogenesis

Background:

  • Arthrobotrys oligospora is a nematode-trapping fungus.
  • Sho1 and Opy2 are key components of the MAPK cascade, vital for fungal stress response.

Purpose of the Study:

  • To investigate the roles of AoSho1 and AoOpy2 in Arthrobotrys oligospora.
  • To understand their involvement in fungal growth, stress response, and pathogenicity.

Main Methods:

  • Gene knockout of Aosho1 and Aoopy2.
  • Phenotypic analysis.
  • Multi-omics approaches (transcriptomics and metabolomics).

Main Results:

  • Knockout mutants showed reduced mycelial growth, spore production, trap formation, and nematode predation.
  • Deletion mutants exhibited increased autophagy and osmotic stress sensitivity.
  • Transcriptome and metabolome data revealed involvement in growth, development, adaptation, and metabolic pathways.

Conclusions:

  • AoSho1 and AoOpy2 are essential for mycelial growth, osmoregulation, and pathogenicity in A. oligospora.
  • These findings provide insights into the MAPK signaling pathway in nematode-trapping fungi.
Keywords:
Nematode-trapping abilityOsmoregulationSecondary metabolismSporulation

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