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Ex Situ Abdominal Multivisceral Normothermic Machine Perfusion: A Research Protocol.

L Leonie van Leeuwen1, Matthew L Holzner1, Kimberly Feeney1

  • 1Organ Perfusion Unit, Recanati/Miller Transplantation Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.

Artificial Organs
|December 3, 2025
PubMed
Summary

Ex situ multivisceral perfusion preserves abdominal organs by mimicking in vivo conditions. This study details a protocol for normothermic machine perfusion, advancing organ preservation and multivisceral transplantation potential.

Keywords:
multivisceral transplantationnormothermic machine perfusionorgan preservationorgan transplantation

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Area of Science:

  • Transplantation Biology
  • Surgical Innovation

Background:

  • Isolated organ perfusion has limitations in replicating abdominal organ interactions.
  • Ex situ multivisceral perfusion offers a more physiological model for preserving multiple organs.

Purpose of the Study:

  • To present a detailed protocol for normothermic machine perfusion of porcine multivisceral grafts.
  • To support preclinical research and translational efforts in multivisceral transplantation.

Main Methods:

  • Development of a stepwise protocol for normothermic machine perfusion.
  • Utilizing porcine abdominal multivisceral grafts for experimental setup.
  • Monitoring key indicators: graft appearance, hemodynamics, biochemistry, and histology.

Main Results:

  • Established a comprehensive perfusion protocol.
  • Identified key indicators of successful perfusion, including stable flow, minimal edema, and lactate clearance.
  • Characterized expected changes during perfusion, such as bowel distension.

Conclusions:

  • Normothermic machine perfusion is a viable strategy for multivisceral graft preservation.
  • The presented protocol can guide future research and clinical applications in transplantation.
  • This method holds promise for improving outcomes in multivisceral transplant recipients.