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Ex Situ Abdominal Multivisceral Normothermic Machine Perfusion: A Research Protocol
L Leonie van Leeuwen1, Matthew L Holzner1, Kimberly Feeney1
1Organ Perfusion Unit, Recanati/Miller Transplantation Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Artificial Organs
|December 3, 2025
Summary
Ex situ multivisceral perfusion preserves abdominal organs by mimicking in vivo conditions. This study details a protocol for normothermic machine perfusion, advancing organ preservation and multivisceral transplantation potential.
Area of Science:
- Transplantation Biology
- Surgical Innovation
Background:
- Isolated organ perfusion has limitations in replicating abdominal organ interactions.
- Ex situ multivisceral perfusion offers a more physiological model for preserving multiple organs.
Purpose of the Study:
- To present a detailed protocol for normothermic machine perfusion of porcine multivisceral grafts.
- To support preclinical research and translational efforts in multivisceral transplantation.
Main Methods:
- Development of a stepwise protocol for normothermic machine perfusion.
- Utilizing porcine abdominal multivisceral grafts for experimental setup.
- Monitoring key indicators: graft appearance, hemodynamics, biochemistry, and histology.
Main Results:
- Established a comprehensive perfusion protocol.
- Identified key indicators of successful perfusion, including stable flow, minimal edema, and lactate clearance.
- Characterized expected changes during perfusion, such as bowel distension.
Conclusions:
- Normothermic machine perfusion is a viable strategy for multivisceral graft preservation.
- The presented protocol can guide future research and clinical applications in transplantation.
- This method holds promise for improving outcomes in multivisceral transplant recipients.
