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Research Domains Environmental Sciences Pollution and Contamination Pollution and contamination not elsewhere classified
Research Domains
Environmental Sciences
Pollution and Contamination
Pollution and contamination not elsewhere classified
Synthesized double stranded gene fragments are not suitable for qPCR endogenous control spike-ins

Synthesized double stranded gene fragments are not suitable for qPCR endogenous control spike-ins

Nathan Zeinstra ¹, Tzitziki Loeza-Quintana ^2,3, Cameron Brown ², Robert Hanner ²

Nathan Zeinstra ¹, Tzitziki Loeza-Quintana ^2,3, Cameron Brown ²

¹Department of Integrative Biology, University of Guelph, Guelph, ON, Canada. nzeinstr@uoguelph.ca.
²Department of Integrative Biology, University of Guelph, Guelph, ON, Canada.
³SLR Consulting LTD, Guelph, ON, Canada.

⁰Department of Integrative Biology, University of Guelph, Guelph, ON, Canada. nzeinstr@uoguelph.ca.

Bmc Research Notes +

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December 3, 2025

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December 3, 2025

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Summary

No abstract available on PubMed

Keywords:

Endogenous control Environmetal DNA Positive control Quantitative PCR Synthetic DNA fragments

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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