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Updated: Jan 9, 2026
10:27
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
Published on: December 5, 2019
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Rice SINA E3 ligases dichotomously control ERECTA1 ubiquitination and stability to regulate panicle morphogenesis and
Zi-Qi Lu1, Tao Guo2, Ying-Jie Cao3
1State Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Molecular Plant
|December 4, 2025
Summary
Rice panicle development is regulated by OsER1. SEVEN IN ABSENTIA OF RICE (SINAR) E3 ligases control OsER1 stability, impacting grain yield. Manipulating SINAR ligases enhances rice yield by optimizing OsER1 signaling.
Area of Science:
- Plant Biology
- Molecular Genetics
- Agricultural Science
Background:
- Rice panicle architecture is crucial for grain yield, influenced by the receptor-like kinase OsER1.
- The precise mechanisms by which OsER1 fine-tunes panicle development are not fully understood.
Purpose of the Study:
- To elucidate the role of SEVEN IN ABSENTIA OF RICE (SINAR) E3 ubiquitin ligase family in regulating rice panicle architecture.
- To investigate how SINAR ligases modulate OsER1 stability and signaling to affect grain yield.
Main Methods:
- Investigated the interactions and functions of SINAR E3 ligase family members in rice.
- Analyzed the ubiquitination and degradation of OsER1 mediated by SINAR ligases.
- Assessed the impact of manipulating SINAR ligase activity on rice panicle development and grain yield.
Main Results:
- SINAR E3 ligases regulate rice spikelet number through synergistic or antagonistic modulation of OsER1 ubiquitination and stability.
- SINAR1 and SINAR6 act as molecular brakes, forming heterodimers to inhibit the ubiquitination activity of SINAR2/3/4/5, thus protecting OsER1 from degradation.
- Targeted suppression of SINAR1 and SINAR6 enhances grain yield by promoting OsER1 degradation, optimizing panicle architecture without compromising fertility.
Conclusions:
- SINAR1 and SINAR6 are key components of a negative feedback loop ensuring optimal OsER1 signaling and panicle architecture.
- An antagonistic, ligase-mediated regulatory mechanism maintains OsER1 receptor homeostasis.
- Manipulation of dichotomous SINAR E3 ligases offers a strategy for enhancing rice yield.
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