Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural characterization and antitumor activity via immune modulation of an α-glucan from Grifola frondosa.

Carbohydrate polymers·2026
Same author

ROS homeostasis in cell fate, pathophysiology, and therapeutic interventions.

Molecular biomedicine·2025
Same author

Heme oxygenase 1 (HO-1) is a drug target for reversing cisplatin resistance in non-small cell lung cancer.

Journal of advanced research·2025
Same author

Intratumoral Collagen Deposition Supports Angiogenesis Suggesting Anti-angiogenic Therapy in Armored and Cold Tumors.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Mitochondrial Gene Scoring System: Predicting Prognosis and Precision Therapy for TACE Non-Response in Hepatocellular Carcinoma Patients.

Journal of Cancer·2025
Same author

Proportion of foetal and placental implantation abnormalities in Madagascar: A cross-sectional study of 35,919 women at public-sector primary healthcare facilities in central and southern Madagascar, 2017-2020.

PloS one·2024

Related Experiment Video

Updated: Jan 9, 2026

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.1K

Prognostic model for osteosarcoma: RNA diagnostics, tumor microenvironment and immunotherapy response.

Ding-Chao Rong1, Xu Rong2, Yuan-Shen Chen3

  • 1Department of Orthopedics, The First Affiliated Hospital of Shaoyang University, Shaoyang, Hunan, 422000, People's Republic of China.

Journal of Orthopaedics
|December 4, 2025
PubMed
Summary

We developed two prognostic models for osteosarcoma, one using 5 small nucleolar RNAs (snoRNAs) and another integrating snoRNAs, lncRNAs, and mRNAs. These models predict patient outcomes and response to therapies like simvastatin.

Keywords:
Drug sensitivityImmune microenvironmentOsteosarcomaPrognostic modelSnoRNA

More Related Videos

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.6K
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.6K

Related Experiment Videos

Last Updated: Jan 9, 2026

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.1K
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.6K
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.6K

Area of Science:

  • Oncology
  • Molecular Biology
  • Bioinformatics

Background:

  • Osteosarcoma is an aggressive cancer with poor prognosis, necessitating accurate prognostic models.
  • Small nucleolar RNAs (snoRNAs) are stable molecules with potential as cancer biomarkers.

Purpose of the Study:

  • To establish reliable prognostic models for osteosarcoma.
  • To identify biomarkers correlating with the tumor immune microenvironment.
  • To predict drug sensitivity in osteosarcoma patients.

Main Methods:

  • Downloaded and analyzed gene expression and clinical data from TCGA, GTEx, and GEO databases.
  • Performed survival analysis, differential gene expression, functional enrichment, and immune cell infiltration analysis.
  • Constructed snoRNA-based and integrated RNA network-based prognostic models, validated drug sensitivity and gene expression in vitro.

Main Results:

  • A 5-snoRNA prognostic model (SNORA2B, SNORA12, SNORD99, SNORD123, SNORD11B) demonstrated prognostic accuracy.
  • An integrated RNA model (PARD6G-AS1, DLX2, TPD52, GRAMD1B) correlated with the immune microenvironment.
  • Both models showed consistent drug sensitivity predictions; osteosarcoma cells were sensitive to simvastatin in vitro.

Conclusions:

  • Independent snoRNAs serve as prognostic biomarkers for osteosarcoma.
  • Integrated RNA models provide insights into the immune microenvironment and immune function.
  • The study offers valuable prognostic and immune-related biomarkers for osteosarcoma, aiding therapeutic strategies.