Systemic microRNA Delivery Using Polysaccharide-Coated Nanobubbles for Ultrasound-Mediated Therapy of Triple-Negative...

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Oncology

Background:

• Triple-negative breast cancer (TNBC) is highly aggressive with poor outcomes.
• Effective nucleic acid delivery to tumors, especially deep within the tumor microenvironment, is a significant challenge.
• Ultrasound-responsive nanobubbles (NBs) show promise for overcoming delivery barriers.

Purpose of the Study:

• To develop a versatile method for loading nucleic acids onto stable, anionic NBs.
• To evaluate the efficacy of methyl glycol chitosan-coated NBs (MGC-NBs) for systemic nucleic acid delivery.
• To assess the therapeutic potential of delivering microRNA-145 using MGC-NBs in TNBC models.

Main Methods:

• Anionic NBs were coated with the cationic polysaccharide methyl glycol chitosan (MGC).
• Nucleic acids were loaded onto the surface of the MGC-NBs.
• The MGC-NBs were tested for systemic delivery in tumor-bearing mouse models.
• The therapeutic effect of delivering microRNA-145 was evaluated in TNBC models.

Main Results:

• A simple and versatile method for loading nucleic acids onto MGC-NBs was established.
• MGC-NBs demonstrated utility as systemic nucleic acid delivery tools.
• The delivery of microRNA-145 using MGC-NBs showed potential for TNBC therapy.

Conclusions:

• MGC-NBs provide a stable and effective platform for nucleic acid delivery.
• This approach offers a promising strategy for overcoming challenges in targeting deep tumor cells.
• The MGC-NB system holds potential for advancing therapeutic strategies against triple-negative breast cancer.