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Related Experiment Video

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Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

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Published on: June 4, 2014

Scoping chromatin dynamics across cortical neurogenesis.

Nicholas K Haghani¹, Megan Y Dennis¹

¹Department of Biochemistry & Molecular Medicine, Genome Center, and MIND Institute, University of California Davis, Davis, CA 95616, USA.

|December 4, 2025

Summary

A new low-input method, SCOPE-C, captures gene regulation during brain development. It reveals cell-type-specific and human-biased regulatory hubs in the developing cortex.

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Area of Science:

Neuroscience
Genomics
Developmental Biology

Background:

Profiling dynamic gene regulatory changes during brain development is complex.
Existing methods lack the resolution or sensitivity for comprehensive analysis.

Purpose of the Study:

To introduce SCOPE-C, a novel low-input method for high-resolution profiling of gene regulation.
To simultaneously capture open chromatin and cis-regulatory contacts during corticogenesis.

Main Methods:

SCOPE-C (Simultaneous Capture of Open chromatin and cis-regulatory contacts) is a low-input technique.
The method was applied to study dynamic regulatory changes during human and mouse corticogenesis.

Main Results:

SCOPE-C enables detailed analysis of gene regulatory landscapes.
The study identified cell-type-specific regulatory hubs.
Human-biased regulatory hubs were discovered during corticogenesis.

Conclusions:

SCOPE-C is an effective tool for studying dynamic gene regulation in brain development.
The findings provide insights into the molecular mechanisms underlying human corticogenesis and its evolutionary aspects.