Atomic force microscope protocols for characterising the elastoviscoplastic biomechanical properties of corneocytes

Area of Science:

• Biophysics
• Materials Science
• Dermatology

Background:

• Corneocytes are key to skin barrier function.
• Previous studies reported variable mechanical properties of corneocytes.
• Artefacts in experimental methods may cause this variability.

Purpose of the Study:

• To establish a reliable method for measuring corneocyte biomechanics.
• To develop a consistent material model for corneocytes.
• To investigate the elastic and inelastic behavior of corneocytes.

Main Methods:

• Utilized Atomic Force Microscopy (AFM) for topographical and mechanical analysis.
• Developed a detailed protocol to mitigate experimental artefacts.
• Employed standard sharp AFM probes for accurate measurements.
• Applied the Herschel-Bulkley model for data interpretation.

Main Results:

• Corneocytes exhibit topographical and biomechanical properties.
• Young's moduli of dry corneocytes resemble glassy organic polymers.
• Corneocytes display viscoplastic behavior.

Conclusions:

• The refined AFM protocol ensures accurate corneocyte characterization.
• Understanding corneocyte mechanics advances skin biomechanics.
• Findings may inform biomimetic material design and dermatological research.