Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antiepileptic Drugs: Glutamate Antagonists01:14

Antiepileptic Drugs: Glutamate Antagonists

863
Glutamate is a fundamental neurotransmitter in the central nervous system, playing a vital role in neuronal communication and various cognitive processes. Glutamate stands as the principal excitatory neurotransmitter in the brain. Its presence is crucial for the communication between neurons, underpinning essential processes such as synaptic transmission, neuronal excitability, and plasticity. These functions are vital for higher-order cognitive processes, including learning and memory. The...
863

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

WONOEP XVII appraisal: The role of the extracellular matrix in epilepsy.

Epilepsia·2026
Same author

Transcriptional profiles of immature neurons in aged human hippocampus track Alzheimer's pathology and cognitive resilience.

Cell stem cell·2026
Same author

Large-scale exome analyses reveal new rare variant contributions in amyotrophic lateral sclerosis.

Nature genetics·2026
Same author

Tau pathology in epilepsy: emerging mechanisms and translational opportunities.

Brain : a journal of neurology·2026
Same author

The clinical value of [<sup>18</sup>F]-fluoro-ethyl-L-tyrosine PET ([<sup>18</sup>F]FET-PET) correlated with MRI in patients with functioning pituitary adenomas: an observational cohort study.

Pituitary·2026
Same author

A novel ketogenic diet that reduces seizures and prevents liver steatosis leads to related gut microbiome changes and restores cecal short-chain fatty acid levels in the rapid kindling rat model of epileptogenesis.

Gut microbes reports·2026

Related Experiment Video

Updated: Jan 9, 2026

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures
10:05

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures

Published on: March 18, 2021

7.5K

Dynamic astrocytic complement C3 activation in the epileptic hippocampus.

Mi Jiang1,2, Alessia Romagnolo3, Eleonora Aronica3

  • 1Department of Neurology, The Third Xiangya Hospital, Central South University Xiangya Medical School, Changsha, China.

Frontiers in Neurology
|December 5, 2025
PubMed
Summary

Complement C3 is upregulated in epilepsy, particularly in reactive astrocytes within the hippocampus. This study reveals the specific timing and location of C3 expression changes during epileptogenesis.

Keywords:
C3neuroinflammationstatus epilepticusstratum lacunosum-molecularetemporal lobe epilepsy

More Related Videos

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins
09:07

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

Published on: August 15, 2017

12.5K
In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy pCLE of Hippocampal Capillaries in Awake Mice
09:08

In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy pCLE of Hippocampal Capillaries in Awake Mice

Published on: April 21, 2023

1.2K

Related Experiment Videos

Last Updated: Jan 9, 2026

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures
10:05

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures

Published on: March 18, 2021

7.5K
Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins
09:07

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

Published on: August 15, 2017

12.5K
In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy pCLE of Hippocampal Capillaries in Awake Mice
09:08

In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy pCLE of Hippocampal Capillaries in Awake Mice

Published on: April 21, 2023

1.2K

Area of Science:

  • Neuroscience
  • Immunology

Background:

  • Complement C3 is implicated in neuroinflammation and upregulated in mesial temporal lobe epilepsy (mTLE).
  • The precise temporal and spatial expression patterns of C3 in the epileptic brain are not fully understood.

Purpose of the Study:

  • To investigate the temporal and spatial expression of complement C3 in the epileptic brain.
  • To characterize C3 dysregulation during epileptogenesis in a mouse model.

Main Methods:

  • RNA sequencing of human mTLE hippocampal tissues.
  • Analysis of GEO database microarray data from an mTLE mouse model.
  • Utilizing a novel floxed C3-IRES-Tdtomato knock-in mouse line for detailed expression analysis.

Main Results:

  • Confirmed C3 upregulation in mTLE hippocampal tissues and mouse models.
  • Identified sustained C3 overexpression primarily in reactive astrocytes in the hippocampal stratum lacunosum-moleculare.
  • Observed C3 activation starting 3 days post-status epilepticus and extending to the CA1 region by 3 weeks.

Conclusions:

  • Complement C3 exhibits region- and stage-specific dysregulation during epileptogenesis.
  • Reactive astrocytes are a key source of sustained C3 overexpression in the epileptic hippocampus.
  • Findings provide insights into the role of C3 in mTLE pathogenesis.