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Related Concept Videos

The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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Lagging Strand Synthesis01:59

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During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
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DNA Replication02:40

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
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ATP and Macromolecule Synthesis01:28

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Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
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Replication in Prokaryotes02:35

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Replication in Prokaryotes01:32

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DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
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Mechanism for a molecular assembler of sequence-controlled polymers using parallel DNA and a DNA polymerase.

Jonathan Bath1,2, Andrew J Turberfield1,2

  • 1Kavli Institute for Nanoscience Discovery, Dorothy Crowfoot Hodgkin Building, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. jonathan.bath@physics.ox.ac.uk.

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Summary

This study introduces a novel DNA-based molecular assembler for programmed chemical reactions. This innovation enables the assembly and evolution of functional polymers, overcoming key challenges in the field.

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Area of Science:

  • Molecular biology
  • Synthetic biology
  • Biochemistry

Background:

  • Constructing molecular assemblers from DNA is crucial for synthesizing functional polymers.
  • Current limitations hinder the programmed execution of chemical reactions by DNA-based systems.

Purpose of the Study:

  • To present a novel mechanism for DNA-based molecular assembly.
  • To address key challenges impeding the development of programmable molecular assemblers.

Main Methods:

  • Utilizing parallel DNA structures.
  • Employing DNA polymerase for programmed chemical reactions.

Main Results:

  • A mechanism for DNA-based molecular assembly has been successfully presented.
  • Two significant challenges in the field have been addressed.

Conclusions:

  • The developed mechanism offers a pathway towards creating advanced DNA-based molecular assemblers.
  • This work facilitates the assembly and evolution of functional polymers.