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Polysome Profiling in Leishmania, Human Cells and Mouse Testis
Published on: April 8, 2018
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Identification of new protein-coding potential in Leishmania braziliensis using a proteogenomics approach
Aditi Shenoy1, Soumi Chowdhury2, Shubhankar Pawar2
1Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.
Biochimica Et Biophysica Acta. Proteins and Proteomics
|December 5, 2025
Summary
This study used proteogenomics to improve the genome annotation of Leishmania braziliensis, a parasite causing American tegumentary leishmaniasis (ATL). Researchers discovered new genes and corrected existing ones, enhancing our understanding of parasite biology and potential therapeutic targets.
Area of Science:
- Genomics
- Proteomics
- Parasitology
Background:
- American tegumentary leishmaniasis (ATL) is caused by Leishmania (Viannia) species, with complex genomes and pathogenicity.
- Leishmania braziliensis genome annotation is limited by hypothetical proteins and unannotated regions.
Purpose of the Study:
- To refine the genome annotation of Leishmania braziliensis using a proteogenomic approach.
- To identify novel protein-coding genes and correct existing gene models.
Main Methods:
- Integrated high-resolution mass spectrometry data with a custom six-frame translated genome database.
- Utilized stringent database-dependent searches with a 1% false discovery rate.
- Identified genome search-specific peptides (GSSPs) mapping to unannotated regions.
Main Results:
- Discovered 56 novel protein-coding genes and corrected 228 existing gene models in L. braziliensis.
- Identified 1034 unique GSSPs in previously unannotated genomic regions.
- Found novel genes encoding proteins with conserved domains (e.g., MACPF, kinesin K39).
Conclusions:
- Proteogenomics effectively uncovers cryptic protein-coding regions, improving genome annotation beyond traditional methods.
- Refined annotation enhances understanding of L. braziliensis biology, virulence, and host interactions.
- Integrated genomic and proteomic data are crucial for kinetoplastid parasite research, aiding diagnostics and interventions.
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