Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell-mediated Immune Responses

Cell-mediated Immune Responses

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

Antigens Involved in Adaptive Immunity

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...

Cells of the Adaptive Immune Response

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

IL-27-mediated hematopoietic dysregulation exacerbates disease severity in severe fever with thrombocytopenia syndrome virus infection.

Science translational medicine·2026

Same author

MotifLeadDB: A Hierarchical Structural Data Set for Congeneric Ligand Binding Activity Change.

Journal of chemical information and modeling·2026

Same author

Structural basis of the sweat odorant HMHA recognition by the human odorant receptor OR52E8.

Communications biology·2026

Same author

Benchmarking and behavioral characterization of LLM agents for protein design.

bioRxiv : the preprint server for biology·2026

Same author

Ginkgo Datapoints Antibody Developability Competition outcomes: limited model performance and a call for data standardization.

mAbs·2026

Same author

Lineage-matched Oropouche virus mRNA-LNP vaccines confer complete, cross-protective immunity in mice.

mBio·2026

Search research articles

Related Experiment Video

Updated: Jan 9, 2026

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

T-SCAPE: T cell immunogenicity scoring via cross-domain aided predictive engine.

Jeonghyeon Kim¹, Nuri Jung¹, Jayyoon Lee²

¹Department of Chemistry, Seoul National University, Gwanak-gu, Seoul 08826, Republic of Korea.

Science Advances

|December 5, 2025

Summary

This summary is machine-generated.

Predicting T cell immunogenicity is crucial for safer vaccines and therapeutics. A new deep learning framework, T-SCAPE, integrates diverse data to accurately forecast T cell activation and therapeutic antibody immunogenicity.

More Related Videos

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Published on: May 17, 2019

Related Experiment Videos

Last Updated: Jan 9, 2026

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Published on: May 17, 2019

Area of Science:

Immunology
Computational Biology
Biotechnology

Background:

T cell immunogenicity is vital for vaccine and therapeutic protein development.
Predicting immunogenicity is challenging due to limited data and complex biological interactions.
Current methods struggle with comprehensive in silico prediction of T cell responses.

Purpose of the Study:

To develop a novel deep learning framework, T-SCAPE, for predicting T cell immunogenicity.
To integrate diverse immunological data sources for improved predictive accuracy.
To assess T-SCAPE's ability to predict T cell activation and therapeutic antibody immunogenicity.

Main Methods:

Developed T cell immunogenicity scoring via cross-domain aided predictive engine (T-SCAPE), a multidomain deep learning framework.
Employed adversarial domain adaptation to integrate data on MHC presentation, pMHC binding, TCR-pMHC interaction, and T cell activation.
Validated T-SCAPE using leakage-controlled benchmarks for predicting T cell activation and antidrug antibody induction.

Main Results:

T-SCAPE demonstrated exceptional performance in predicting T cell activation for specific peptide-MHC pairs.
The framework accurately predicted the antidrug antibody-inducing potential of therapeutic antibodies without MHC inputs.
Multidomain pretraining based on biological relevance and data significantly contributed to T-SCAPE's success.

Conclusions:

T-SCAPE offers a robust and accurate method for predicting T cell immunogenicity.
The framework's ability to integrate diverse data enhances its predictive power.
T-SCAPE has the potential to significantly advance the development of safer and more effective vaccines and protein therapeutics.