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Related Experiment Video

Updated: Jan 9, 2026

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Rapid biocompatible solid phase microextraction: Nirogacestat protein binding in post dose human serum samples.

Helen Patten1, Rochelle Burke1, Sohrab Habibi Goudarzi1

  • 1KCAS Bio, 10830 S Clay Blair Blvd. Olathe, Kansas, USA 66061.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|December 5, 2025
PubMed
Summary
This summary is machine-generated.

Biocompatible solid phase microextraction (BioSPME) offers a superior method for measuring drug protein binding compared to rapid equilibrium dialysis (RED). This adsorption-based technology accurately quantifies free drug concentrations in clinical samples, overcoming limitations of traditional techniques.

Keywords:
Adsorption-based protein bindingBiocompatible solid phase microextractionLC-MS/MSNirogacestatProtein bindingRapid equilibrium dialysis

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Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Biotechnology

Background:

  • Protein binding significantly impacts drug permeation and efficacy, as only unbound drugs exert pharmacological effects.
  • Traditional methods like rapid equilibrium dialysis (RED) for quantifying free drug concentrations face challenges including equilibration time, drug instability, and non-specific binding.
  • Nirogacestat exhibited non-specific binding with RED, necessitating a more accurate protein binding assessment method.

Purpose of the Study:

  • To evaluate biocompatible solid phase microextraction (BioSPME) as an alternative method for accurately measuring nirogacestat protein binding.
  • To compare the efficacy of BioSPME against RED for protein binding assessment in both in vitro and clinical samples.

Main Methods:

  • Utilized adsorption-based biocompatible solid phase microextraction (BioSPME) technology.
  • BioSPME employs a biocompatible sorbent to selectively extract unbound analytes from sample matrices.
  • Controlled incubation times and container types were managed during the BioSPME process.

Main Results:

  • BioSPME demonstrated accurate and reliable measurement of nirogacestat protein binding in both in vitro and clinical samples.
  • The method effectively overcomes the non-specific binding issues encountered with RED for nirogacestat.
  • Achieved precise quantification of protein binding, essential for understanding drug pharmacokinetics.

Conclusions:

  • BioSPME is a suitable and accurate alternative to RED for assessing drug protein binding, particularly for highly bound drugs like nirogacestat.
  • This method provides reliable protein binding data from clinical serum samples.
  • BioSPME enhances the accuracy of free drug concentration measurements, crucial for drug development and clinical application.