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Updated: Jan 9, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Stress-tolerant heterotrophic nitrification-aerobic denitrification strains: nitrogen removal performance,
Heng Wu1, Guoqiang Zhan1, Huaiwen Zhang2
1Agricultural Microbial Agents Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, PR China.
Abstract:
Stress-resistant heterotrophic nitrification-aerobic denitrification (HN-AD) strains can efficiently remove nitrogen from wastewater with high ammonia, high acidity, high salinity, low temperature, and heavy metals. This study systematically reviews the nitrogen removal characteristics, application performance, metabolic mechanisms and future prospects of stress-resistant HN-AD strains. As a result, (1) Stress-resistant HN-AD strains have gained growing interest, especially multi-stress-resistant strains. (2) HN-AD genera Acinetobacter (resistant to high ammonia and low temperature) and Pseudomonas (resistant to high ammonia and salinity) can improve bioreactors' tolerance, requiring further engineering applications. (3) Stress-resistant HN-AD strains resist stress via three mechanisms: upregulating nitrogen metabolism genes, enhancing membrane transport, and modulating signaling molecules, which results in enhanced bioreactor performance. (4) Screening multi-stress-resistant strains should remain a priority, focusing on large-scale application, conductive material immobilization, colonization and genetic mechanism verification, and life cycle/economic analysis. This study provides vital references for HN-AD strain screening and applications under extreme conditions.
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