qPCR-melting curve analysis in molecular diagnostics

Melting curve analysis following qPCR allows for the efficient and cost-effective detection of multiple targets. By examining the shape and number of peaks in the melting curve, multiple targets can be simultaneously detected within a single tube. The integration of melting curve analysis with various probe technologies continues to advance the capabilities of molecular testing in pathogen detection. This paper reviews the principles and development of qPCR-melting curve analysis in molecular diagnostics and explores future trends in this analytical technique.