Diclofop-methyl resistance conferred by LrGSTU181 overexpression in Lolium rigidum
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View abstract on PubMed
Summary
This summary is machine-generated.The study identifies LrGSTU181, a glutathione S-transferase (GST), as the key enzyme conferring resistance to the herbicide diclofop-methyl in Lolium rigidum. This discovery expands our understanding of metabolic resistance mechanisms in this significant agricultural weed.
Area Of Science
- Plant Science
- Biochemistry
- Agricultural Science
Background
- Lolium rigidum poses a significant threat to the global agricultural economy due to increasing herbicide resistance.
- The Australian L. rigidum population WALR70 exhibits non-target-site resistance (NTSR) to diclofop-methyl, necessitating investigation into its underlying mechanisms.
Purpose Of The Study
- To elucidate the specific resistance mechanisms conferring diclofop-methyl tolerance in the Australian L. rigidum population WALR70.
- To identify and functionally characterize the genes and enzymes involved in this non-target-site resistance.
Main Methods
- Comparative analysis of enzyme activity between resistant (WALR70) and susceptible (SVLR1) populations.
- Gene expression profiling using RNA-sequencing (RNA-seq) and quantitative real-time PCR (RT-qPCR).
- Functional validation of candidate genes (LrGSTU181 and UGT79) through heterologous expression in yeast and rice, and molecular docking studies.
Main Results
- Glutathione S-transferase (GST) enzyme activity was significantly higher (2.8-fold) in the resistant population.
- A tau class GST gene, LrGSTU181, was found to be significantly upregulated in the resistant population.
- LrGSTU181-transformed yeast and rice exhibited enhanced tolerance to diclofop-methyl, and molecular docking confirmed high-affinity binding of LrGSTU181 to diclofop acid.
Conclusions
- The tau class glutathione S-transferase LrGSTU181 is functionally established as a mediator of diclofop-methyl resistance in Lolium rigidum.
- This finding expands the known metabolic basis of non-target-site resistance (NTSR) in this economically important weed species.
- Understanding LrGSTU181's role provides potential targets for managing diclofop-methyl resistance in agriculture.
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