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Uricase-free and cost-effective colorimetric assay for uric acid based on CoBTC metal-organic frameworks
Shokooh Sadat Khaloo1,2, Mohammad Hossein Vaziri3,4, Fatemeh Azizi5
1Workplace Health Promotion Research Center, Research Institute for Health Sciences and Environment, Shahid Beheshti University of Medical Sciences, Tehran, Iran. sh_khaloo@sbmu.ac.ir.
Abstract:
The development of enzyme-free uric acid (UA) detection methods remains challenging due to cost, stability, and sensitivity limitations. Here, we report a uricase-free colorimetric biosensor using cobalt-1,3,5-benzene tricarboxylate metal-organic frameworks (CoBTC MOFs) with intrinsic peroxidase-like activity. CoBTC catalyzes the oxidation of TMB by conducting H₂O₂ to blue oxTMB, while UA reduces oxTMB to colorless TMB, allowing for the quantification of UA via absorbance changes. This biosensor achieved a detection limit of 0.25 μM and a linear range of 0.4-180 μM, outperforming many uricase-dependent assays. Clinical validation with human serum (recovery: 98-103%, RSD < 3%) confirmed the resistance to matrix effects. This work provides a scalable and low-cost alternative for point-of-care UA monitoring in diagnostic settings.
