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Updated: Jan 9, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
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Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

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CircFOXK2 induces non-small cell lung cancer tumorigenesis through the miR-328-5p/PKP3 axis

Tongwei Xiang1, Liping Chen2, Huaying Wang3

  • 1Department of Respiratory and Critical Care Medicine, Yinzhou People's Hospital, The Affiliated People's Hospital of Ningbo University, Ningbo, China. 603358673@qq.com.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|December 6, 2025
PubMed

Insights

Circular RNAs (circRNAs) promote non-small cell lung cancer (NSCLC) progression. This study reveals circFOXK2 enhances NSCLC by sponging miR-328-5p, upregulating PKP3, highlighting a novel therapeutic pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • RNA Biology

Background:

  • Circular RNAs (circRNAs) are regulatory non-coding RNAs (ncRNAs) involved in cancer development.
  • The specific role and molecular mechanisms of circFOXK2 in non-small cell lung cancer (NSCLC) remain largely unelucidated.

Purpose of the Study:

  • To investigate the functional role of circFOXK2 in NSCLC progression.
  • To elucidate the molecular mechanism by which circFOXK2 influences NSCLC tumorigenesis, focusing on the miR-328-5p/PKP3 signaling pathway.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess expression levels of circFOXK2, miR-328-5p, and PKP3.
  • Cellular assays including CCK-8 for proliferation, wound healing for migration, and Transwell assays for invasion.
  • Bioinformatic analysis and dual-luciferase reporter assays (DLRGAs) to confirm interactions between miR-328-5p and circFOXK2.

Main Results:

  • circFOXK2 and PKP3 were upregulated, while miR-328-5p was downregulated in NSCLC tissues and cell lines.
  • circFOXK2 significantly promoted NSCLC cell proliferation, migration, and invasion in vitro.
  • circFOXK2 functions as a molecular sponge for miR-328-5p, leading to the upregulation of its target gene, PKP3.

Conclusions:

  • circFOXK2 drives NSCLC progression through the miR-328-5p/PKP3 signaling axis.
  • This circRNA-mediated pathway represents a potential therapeutic target for non-small cell lung cancer treatment.

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