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A New Rapid Method for Detecting Trace Nitrite Using Ultrasound Assistance
1School of Chemical Engineering and Materials, Changzhou Institute of Technology, Changzhou, 213032, P.R. China. jiangfh@czu.cn.
Journal of Fluorescence
|December 6, 2025
Summary
A new method uses the fluorescent probe ANDSA for rapid, sensitive nitrite detection in environmental samples. This technique requires minimal reagents and ultrasound assistance, achieving results in just 12 minutes.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Spectroscopy
Background:
- Trace-level nitrite detection is crucial for environmental monitoring.
- Existing methods may be time-consuming or require extensive reagents.
- Developing rapid and sensitive nitrite detection is an ongoing challenge.
Purpose of the Study:
- To develop a rapid, reagent-minimal, and highly sensitive method for trace nitrite detection.
- To utilize the spectral characteristics of 6-amino-1,3-naphthalenedisulfonic acid (ANDSA) for nitrite quantification.
- To optimize the method for practical application in environmental water samples.
Main Methods:
- Utilized 6-amino-1,3-naphthalenedisulfonic acid (ANDSA) as a fluorescent probe.
- Employed ultrasound assistance to accelerate the detection process.
- Optimized reagent concentrations (sulfuric acid, potassium bromide) and excitation/emission wavelengths (278 nm / 465 nm).
Main Results:
- Established a sensitive detection method for trace nitrite based on ANDSA fluorescence quenching.
- Achieved a detection time of 12 minutes with ultrasound assistance.
- Demonstrated a strong exponential correlation between nitrite concentration and ANDSA fluorescence response (F₀/F = 0.94183 × e^(0.8766 C)).
- Obtained low relative standard deviations (0.4%-2.5%) and high recovery rates (89.87%-101.97%) in real water samples.
Conclusions:
- The developed method offers a highly sensitive and rapid approach for trace nitrite detection.
- The reagent-minimal protocol makes it suitable for field applications.
- This technique provides a reliable tool for environmental nitrite monitoring.
