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MHASS: Microbiome HiFi Amplicon Sequencing Simulator.

Rye Howard-Stone¹, Ion I Măndoiu¹

¹School of Computing, University of Connecticut, CT, 06269, United States.

Bioinformatics (Oxford, England)

|December 6, 2025

Summary

This summary is machine-generated.

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The Microbiome HiFi Amplicon Sequence Simulator (MHASS) generates realistic synthetic PacBio HiFi amplicon sequencing data for microbiome research. This tool aids in validating and benchmarking long-read analysis workflows for microbial community studies.

Area of Science:

Bioinformatics
Microbiology
Genomics

Background:

Accurate microbiome analysis relies on high-quality sequencing data.
Simulating realistic amplicon sequencing data is crucial for tool development and validation.

Purpose of the Study:

To introduce the Microbiome HiFi Amplicon Sequence Simulator (MHASS).
To provide a tool for generating synthetic PacBio HiFi amplicon sequencing datasets for microbiome studies.

Main Methods:

MHASS integrates genome-aware abundance modeling.
It incorporates realistic dual-barcoding strategies.
Empirically derived pass-number distributions from actual sequencing runs are used.

Main Results:

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Last Updated: Jul 1, 2026

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Published on: October 15, 2019

MHASS generates realistic synthetic PacBio HiFi amplicon sequencing datasets.

The datasets are suitable for rigorous benchmarking of microbiome analysis workflows.

Conclusions:

MHASS enables the validation of long-read microbiome analysis workflows.
It supports the accurate ASV clustering and taxonomic assignment in microbiome studies.