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Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

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Point-of-Need PFAS Detection: A Yes/No Biosensor Solution.

Henry F F Bellette^1,2, Dênio E Pires Souto³, Alexandre Xavier Mendes^4,5

¹The Biomedical and Environmental Sensor Technology (BEST) Research Centre, Biosensors Program, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, Victoria 3086, Australia.

|December 6, 2025

Summary

A new protein-based electrochemical sensor offers a field-deployable solution for detecting perfluorooctanoic acid (PFOA), a harmful chemical. This affordable device can monitor PFOA in environmental and biological samples, addressing a critical need for accessible PFAS testing.

Keywords:

PFAS antifouling coatings environmental monitoring fatty acid binding protein lubricin point-of-need electrochemical sensor

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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Area of Science:

Environmental Chemistry
Biomedical Engineering
Analytical Chemistry

Background:

Per- and polyfluoroalkyl substances (PFAS) are widespread environmental contaminants with significant health risks.
Current detection methods like LC/MS/MS are costly, time-consuming, and require specialized labs.
There is an urgent need for portable, user-friendly devices for PFAS monitoring.

Purpose of the Study:

To develop a protein-based electrochemical sensor for rapid, point-of-need detection of perfluorooctanoic acid (PFOA).
To demonstrate the sensor's efficacy in detecting PFOA in various sample matrices.

Main Methods:

Utilized two proteins: lubricin (LUB) and engineered human liver fatty acid binding protein with a methylene blue redox tag (FABP1-MB).
Developed an electrochemical sensing platform for PFOA detection.
Validated sensor performance using water and whole blood samples.

Main Results:

Achieved PFOA detection at concentrations of 0.41 ng/L and 0.41 μg/L.
Successfully demonstrated PFOA detection in real-world water samples.
Showcased PFOA detection in whole blood samples, indicating biomedical monitoring potential.

Conclusions:

The developed protein-based electrochemical sensor provides a promising tool for PFOA detection.
This technology offers a field-deployable, accessible alternative to traditional analytical methods.
The sensor has potential applications in both environmental surveillance and clinical diagnostics.