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An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Published on: August 18, 2018
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A streamlined protocol for high-quality RNA extraction from bread dough
Akira Nishimura1, Shigeyuki Ota2, Shota Isogai3
1Department of Food and Agricultural Sciences, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
Journal of Microbiological Methods
|December 6, 2025
Abstract:
Bread dough is rich in polysaccharides and polyphenols, which hinder RNA extraction. We therefore established a streamlined workflow combining Fruit-mate homogenization, clarification, TRIzol extraction, high-salt precipitation, and activated-charcoal cleanup. This protocol reproducibly yields intact RNA of sufficient purity, enabling transcriptomic analyses of yeast activity in complex dough environments.

