Related Experiment Video
Updated: Jan 9, 2026

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
Published on: June 7, 2017
Deconstructing the six-step hand rubbing technique for healthcare staff: a mixed-methods investigation of efficacy,
Lucyna Gozdzielewska1, Kareena McAloney-Kocaman1, Sue Lang2
1SHIP Research Group, Research Centre for Health, Glasgow Caledonian University, Cowcaddens Road, Glasgow, G4 0BA, UK.
Background:
A six-step technique is recommended for applying alcohol-based hand rubs (ABHRs) in health care. However, evidence supporting the effectiveness of individual steps is limited and healthcare workers' (HCWs)' compliance is low, potentially due to low acceptability or feasibility or inadequate training. This study evaluated the effectiveness of the six-step hand rubbing technique for healthcare staff with consideration of microbiological efficacy, acceptability and feasibility.
Methods:
A mixed-method design was used. Data were collected in two stages between 2017 and 2020. Stage 1 used a Latin square design with a modified EN 1500 procedure to compare the six-step technique with six variations omitting one step each, for bacterial load reduction and hand surface coverage (N = 35). Stage 2 was a cross-sectional online survey of the acceptability and feasibility of using the six-step hand rubbing technique in clinical practice (N = 78).
Results:
Omitting step 4 (backs of fingers) resulted in significantly lower bacterial load reduction than with the full six-step technique (P = 0.001). Omitting step 1 (palms) or step 2 (dorsa) resulted in significantly lower hand coverage than with all other technique variations (P ≤ 0.001). Stage 2 participants perceived the six-step technique as acceptable and feasible, with step 1 as the most acceptable and feasible step.
Conclusion:
Step 4 contributed most to bacterial load reduction, and steps 1 and 2 were key for hand coverage. HCWs should continue using the six-step technique, with efforts to improve compliance with individual steps. Future research should investigate the effects of altering step sequence and the contribution of individual steps during handwashing with soap and water.
Related Concept Videos
Handwashing III: During the Procedure and Post-Procedure Steps
Handwashing II: Pre-procedure and Initial Procedure Steps
The hand washing procedure itself includes the following steps. First, cover cuts, if any, on hands with a waterproof dressing. Cuts and abrasions can become contaminated with bacteria hindering the ability to clean the area thoroughly. In addition, repeated hand washing can worsen an injury. The nails must be short and clean, without nail...
Handwashing I: Introduction and Types of Equipment
Hand wash basins in clinical areas should have faucets that can be turned on and off without using the hands; that is, they should be non-touch or lever-operated....
Hand hygiene
Hand washing...
PPE Use in Healthcare Settings II: Doffing
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...

