Related Experiment Video

Updated: Jan 7, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

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CSNK1D inhibition suppresses head and neck squamous cell carcinoma progression through SHH and PTCH1 pathway

Li Yang1,2, Xiang Li3, Yun Zhu1,4

  • 1Department of Oral and Maxillofacial Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Cell Death & Disease
|December 6, 2025
PubMed

Insights

Casein kinase 1 delta (CSNK1D) drives head and neck cancer progression by activating the hedgehog pathway. Inhibiting CSNK1D presents a potential new treatment strategy for HNSCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Casein kinase 1 delta (CSNK1D) is implicated in cancer, but its role in head and neck squamous cell carcinoma (HNSCC) is not well understood.
  • Understanding CSNK1D's function is crucial for developing targeted therapies for HNSCC.

Purpose of the Study:

  • To investigate the biological functions and clinical significance of CSNK1D in HNSCC progression.
  • To elucidate the molecular mechanisms by which CSNK1D regulates HNSCC growth.
  • To evaluate CSNK1D as a potential therapeutic target for HNSCC.

Main Methods:

  • Analysis of CSNK1D expression in HNSCC tumor tissues.
  • Investigation of CSNK1D's interaction with SHH and PTCH1.
  • Assessment of the effect of CSNK1D on the GLI1-BCL2 axis and hedgehog pathway activation.
  • Evaluation of the inhibitory effect of SB-203580 on CSNK1D in HNSCC.

Main Results:

  • CSNK1D expression is upregulated in HNSCC tissues and correlates with poor patient survival.
  • CSNK1D interacts with SHH and PTCH1, stabilizing the complex and activating the GLI1-BCL2 axis.
  • Activation of the hedgehog pathway is mediated by CSNK1D in HNSCC.
  • SB-203580 effectively inhibits CSNK1D activity.

Conclusions:

  • CSNK1D is a critical oncogenic driver in HNSCC progression.
  • CSNK1D regulates HNSCC via the hedgehog pathway.
  • Targeting CSNK1D with inhibitors like SB-203580 offers a promising therapeutic strategy for HNSCC.

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