Broad-Spectrum Antibacterial Activity of Postbiotic From Lacticaseibacillus paracasei BGP1 Against...

Area of Science:

• Microbiology
• Dermatology
• Biotechnology

Background:

• Antibiotic resistance necessitates novel therapeutic strategies for skin wound infections.
• Probiotics offer potential but face formulation challenges with live cells in dermatology.
• Postbiotics present a stable, safe, and nonliving alternative for antimicrobial applications.

Purpose of the Study:

• To evaluate the antibacterial potential of postbiotics derived from probiotic strains, specifically Lacticaseibacillus paracasei BGP1.
• To assess the efficacy of cell-free supernatants (CFSs) against clinically relevant skin pathogens.
• To identify bioactive compounds responsible for the observed antimicrobial activity.

Main Methods:

• Screening of CFSs from six probiotic strains using agar well diffusion and broth microdilution assays.
• Testing against Staphylococcus aureus (ATCC 25923), MRSA (UTMC 1442), Pseudomonas aeruginosa (ATCC 27853), and PAO1.
• Gas Chromatography-Mass Spectrometry (GC-MS) for identification of bioactive compounds.

Main Results:

• Lacticaseibacillus paracasei BGP1 demonstrated significant antibacterial activity with a Minimum Inhibitory Concentration (MIC) of 6.25 mg/mL and a Minimum Bactericidal Concentration (MBC) of 12.5 mg/mL.
• GC-MS analysis identified palmitic acid (33.24%) and stearic acid (46.45%) as the dominant bioactive compounds in the BGP1 CFS.
• The CFS from BGP1 exhibited broad-spectrum activity against the tested skin pathogens.

Conclusions:

• Postbiotic metabolites from L. paracasei BGP1 are effective against antibiotic-resistant skin wound pathogens.
• These findings highlight a promising, stable, and nonliving alternative to conventional antibiotic therapies.
• Further in vivo studies are warranted to explore therapeutic potential and clinical formulation.