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Related Experiment Video

Updated: Jan 9, 2026

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Published on: October 17, 2025

A Bioengineered Skin Organoid Platform for Modeling Human Skin Physiology and Cytotoxicity.

Anastasiya Gorkun-Roeder¹, Naresh Mahajan¹, Gemma Nomdedeu-Sancho¹

¹Wake Forest Institute for Regenerative Medicine.

Research Square

|December 8, 2025

Summary

Researchers created advanced human skin organoids (SOs) using six cell types. These complex SOs mimic native skin structure and function, offering a powerful tool for skin research and disease modeling.

Keywords:

body-on-a-chip chemical irritation high-throughput testing skin development ultraviolet B radiation

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Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

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Last Updated: Jan 9, 2026

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

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Published on: October 17, 2025

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

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Published on: May 17, 2021

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

Area of Science:

Biotechnology
Tissue Engineering
Dermatology

Background:

Current 3D skin models often lack cellular diversity, limiting their physiological relevance.
Existing models typically use only fibroblasts and keratinocytes, omitting crucial cell types.

Purpose of the Study:

To develop a multi-cell type, self-organizing human skin organoid (SO) model.
To create a more physiologically relevant in vitro system for skin research.

Main Methods:

Utilized organoid fabrication technology for self-organization of six primary human skin cell types.
Cultured keratinocytes, melanocytes, dermal papilla cells, endothelial cells, fibroblasts, and adipocytes under non-adhesive, gravity-driven conditions.

Main Results:

Developed SOs exhibiting spontaneous morphogenesis with stratified epidermis and dermal-hypodermal core.
SOs maintained skin-like organization for over 21 days, showing epidermal barrier integrity, retinol metabolism, and responsiveness to UV and toxins.
Demonstrated melanogenesis and vasculogenesis within the SOs.

Conclusions:

A self-organizing, multi-cell type skin organoid system was successfully developed.
The SO model recapitulates key human skin architecture and function in vitro.
This platform is valuable for skin physiology, toxicology, regenerative medicine, and disease modeling.