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Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

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Related Experiment Video

Updated: Jun 27, 2026

Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry LCM-LC/MS
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Targeted LC-MS/MS method for quantifying respiratory pharmaceuticals in wastewater.

Regina L Gasparetto1,2, Scott Bickel3,4, Xinmin Yin1,2

  • 1Department of Chemistry, University of Louisville 2210 South Brook Street Louisville Kentucky 40208 USA xiang.zhang@louisville.edu +1 502 852 8878.

Environmental Science : Water Research & Technology
|December 8, 2025
PubMed
Summary
This summary is machine-generated.

A new liquid chromatography-mass spectrometry (LC-MS)/MS method reliably detects respiratory pharmaceuticals in wastewater. This wastewater-based epidemiology approach supports public health surveillance of medication use.

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Public Health

Background:

  • Wastewater-based epidemiology (WBE) monitors population health by analyzing wastewater.
  • Sensitive methods are needed to detect respiratory medications in wastewater.
  • Existing methods lack sensitivity for complex wastewater matrices.

Purpose of the Study:

  • To develop and validate a sensitive LC-MS/MS method for detecting 10 common respiratory pharmaceuticals in wastewater.
  • To improve wastewater analysis for public health surveillance.

Main Methods:

  • Developed a liquid chromatography-mass spectrometry (LC-MS)/MS method with multiple reaction monitoring.
  • Integrated freeze-drying for preconcentration and online solid-phase extraction for cleanup.
  • Utilized stable isotope-labeled internal standards (SILs) to correct for matrix effects.

Main Results:

  • Achieved low detection and quantification limits (0.7-19 ng L⁻¹ and 3-125 ng L⁻¹).
  • Demonstrated high recoveries (82-194%) and precision (<7.2% RSD).
  • Successfully detected 9 of 10 target compounds in wastewater samples, with 6 above quantification limits.

Conclusions:

  • The validated LC-MS/MS method is robust, high-throughput, and requires low sample volumes.
  • This method enables reliable detection of respiratory drugs in wastewater.
  • Supports WBE for pharmaceutical use surveillance and public health monitoring.