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Related Experiment Video

Updated: Jan 9, 2026

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High-Throughput Targeted Sequencing Identifies an HPV Methylation Panel for Detecting Cervical Lesion Progression.

Hui Liu1, Jie Zhou2, Yunxia Xiao3

  • 1Department of Pathology, Xi'an People's Hospital (Xi'an Fourth Hospital), Affiliated People's Hospital Northwest University, Xi'an, Shaanxi, China.

Journal of Medical Virology
|December 8, 2025
PubMed
Summary

Quantitative methylated HPV DNA markers show promise for precisely detecting cervical lesions. A novel five-CpG methylation panel effectively distinguishes cervical cancer precursors in HPV16-positive women.

Keywords:
HPV methylationcervical cancercervical intraepithelial neoplasiahuman papillomavirus

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • High-risk human papillomavirus (hrHPV) infection is the primary cause of cervical cancer.
  • Current hrHPV testing lacks specificity for detecting neoplastic changes, necessitating improved diagnostic tools.

Purpose of the Study:

  • To explore the utility of quantitative methylated HPV DNA markers for precise detection of cervical lesions.
  • To identify novel HPV16 methylation markers capable of distinguishing cervical lesions from normal tissue.

Main Methods:

  • Genome-wide HPV methylation patterns were analyzed using hybridization capture-based bisulfite sequencing.
  • A training cohort (60 samples) and a validation cohort (29 samples) were utilized.
  • Analysis focused on 112 CpG sites across the HPV genome, correlating methylation levels with disease progression.

Main Results:

  • Genome-wide HPV16 methylation levels correlated significantly with cervical cancer progression (SCC vs. normal tissue, p=0.0032).
  • Progressive methylation increases were observed in E5-α and L2 genes across lesion severity.
  • A five-CpG methylation panel demonstrated high discriminatory performance (AUC=0.919) in a validation cohort for detecting cervical lesions.

Conclusions:

  • Novel HPV16 methylation markers were identified that distinguish cervical lesions from normal tissue.
  • A five-CpG methylation panel shows promise as a triage biomarker for HPV16-positive women in cervical cancer screening.
  • HPV methylation-based triage is feasible for improving cervical cancer screening accuracy.