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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

970
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
970

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Luminescent array sensors for pathogen identification using AIE-active cationic iridium complexes.

Sonali Bera1, Aryan Gautam2, Gaurank Pal1

  • 1Medicinal Chemistry and Chemical Biology Laboratory, Amity Institute of Click Chemistry Research and Studies, Amity University, Noida, Uttar Pradesh 201303, India. pprasad@amity.edu.

Chemical Communications (Cambridge, England)
|December 8, 2025
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Summary

New luminescent probes rapidly identify antibiotic-resistant bacteria, including ESKAPE pathogens, with high accuracy. This technology shows promise for detecting mixed infections in urinary tract infections (UTIs).

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Microbiology

Background:

  • Antibiotic resistance poses a significant global health threat.
  • Rapid and accurate identification of bacterial strains is crucial for effective treatment.
  • Current methods for bacterial identification can be time-consuming.

Purpose of the Study:

  • To develop and evaluate novel luminescent probes for distinguishing antibiotic-sensitive and -resistant bacterial strains.
  • To assess the probes' efficacy in identifying clinically relevant pathogens, including ESKAPE bacteria.
  • To explore the potential of the sensor array for detecting mixed bacterial infections in urine samples.

Main Methods:

  • Synthesis and characterization of five novel aggregation-induced emission (AIE)-active iridium(III) probes.
  • Application of the probes in a sensor array format for bacterial identification.
  • Testing the sensor array with various pathogenic bacterial strains at different concentrations.
  • Validation of results using clinical urine samples with suspected urinary tract infections (UTIs).

Main Results:

  • The AIE-active iridium(III) probes enabled rapid and specific identification of antibiotic-sensitive and -resistant strains.
  • Nearly 100% accuracy was achieved, even at low bacterial concentrations (10^5 CFU mL^-1).
  • The sensor array successfully identified mixed infections in urine samples, demonstrating clinical relevance.

Conclusions:

  • The developed luminescent probes offer a highly accurate and efficient method for bacterial identification.
  • This technology has significant potential for the early diagnosis and management of antibiotic resistance and UTIs.
  • The sensor array provides a promising platform for point-of-care diagnostics.