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Updated: Jan 9, 2026
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Microvascular Embolism Mouse Model for In Vivo Two-photon Microscopy Using Fluorescent Polystyrene Microspheres
Published on: November 21, 2025
251
Microvascular Embolism Mouse Model for In Vivo Two-photon Microscopy Using Fluorescent Polystyrene Microspheres
Kevin Mol1, Judith de Vos1, Sanne Kok2
1Biomedical Engineering and Physics, Amsterdam University Medical Center, University of Amsterdam; Amsterdam Neuroscience, neurovascular disorders; Amsterdam Cardiovascular Sciences.
Journal of Visualized Experiments : Jove
|December 8, 2025
Summary
Researchers developed a new method to study brain microvascular occlusions in mice using advanced imaging. This technique allows for long-term observation of these events and their impact on brain blood flow.
Area of Science:
- Neuroscience
- Cerebrovascular Research
- Medical Imaging
Background:
- Brain microvascular occlusions are common yet poorly understood.
- Existing study models have limitations.
- Long-term consequences and clearance mechanisms are unknown.
Purpose of the Study:
- To develop a robust method for inducing and studying cerebral microvascular embolisms in vivo.
- To enable long-term, high-resolution imaging of micro-occlusions.
- To investigate the impact and clearance dynamics of these events.
Main Methods:
- Developed a protocol combining chronic cranial window preparation with intra-arterial microsphere injection in mice.
- Utilized two-photon microscopy for in vivo imaging of microvasculature and occlusions.
- Validated the injection technique using post-mortem 3D imaging and demonstrated utility with in vivo imaging.
Main Results:
- Established a consistent and robust method for inducing cerebral microvascular embolisms.
- Enabled long-term in vivo imaging of micro-occlusions.
- Demonstrated the microsphere distribution and imaging capabilities.
Conclusions:
- The developed method offers a powerful tool for studying brain microvascular occlusions.
- Facilitates investigation into the impact and clearance of micro-embolisms.
- Advances research in cerebrovascular disease and stroke models.
