Enhancing Desferrioxamine B production by Streptomyces pilosus through morphological and process optimization using...

Area of Science:

• Microbiology
• Biotechnology
• Biochemical Engineering

Background:

• Desferrioxamine B (DFOB) is a vital siderophore with significant therapeutic applications.
• Optimizing DFOB production by Streptomyces pilosus is crucial for meeting clinical demand.
• Previous studies have explored various factors influencing DFOB yield, but record-level productivity remains a target.

Purpose of the Study:

• To enhance Desferrioxamine B (DFOB) yield and productivity from Streptomyces pilosus.
• To optimize fermentation conditions using statistical methods like Response Surface Methodology (RSM) and the Taguchi method.
• To investigate the impact of medium composition and process parameters on DFOB biosynthesis.

Main Methods:

• Response Surface Methodology (RSM) was employed to optimize yeast extract, MgSO4·7H2O concentration, and buffer molarity for flask cultures.
• The Taguchi method (L9 orthogonal array) was utilized in a 2-L bioreactor to optimize glucose, yeast extract, and agitation speed at a controlled pH.
• Field Emission Scanning Electron Microscopy (FESEM) was used to analyze microbial morphology under different agitation rates.

Main Results:

• RSM optimization in flasks yielded 1.55 g/L DFOB (0.63 g/L·day), the highest reported flask productivity.
• Bioreactor optimization using the Taguchi method achieved 2.35 g/L DFOB (0.235 g/L·h), the highest reported bioreactor productivity.
• FESEM revealed that excessive agitation led to mycelial fragmentation, negatively impacting DFOB production.

Conclusions:

• Optimized medium composition and controlled fermentation parameters significantly enhance DFOB production in Streptomyces pilosus.
• The study achieved unprecedented DFOB yields in both flask and bioreactor systems.
• Findings provide a foundation for scaling up DFOB production for industrial applications.