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Lactic acid bacteria: potentials in canine formulas for puppies
Natalia Carrasco1, María Hortencia Miranda1, Cecilia Aristimuño Ficoseco1
1Centro de Referencia para Lactobacilos (CERELA-CONICET-FML-FECIC), San Miguel de Tucumán, Chacabuco 145, CP4000, Argentina.
Veterinary Research Communications
|December 9, 2025
Summary
This study evaluated canine probiotic strains for safety and effectiveness. Four compatible strains, Lactobacillus johnsonii 67, Lactiplantibacillus plantarum 74, Ligilactobacillus salivarius 84, and Pediococcus acidilactici 81, were selected for a new canine probiotic formula.
Area of Science:
- Companion Animal Health
- Microbiology
- Veterinary Probiotics
Background:
- Growing popularity of probiotics and nutraceuticals in veterinary medicine as alternatives to antibiotics.
- Antibiotics pose risks including bacterial resistance and disruption of gut microbial ecology.
- Need for safe and effective probiotic formulations for companion animals.
Purpose of the Study:
- To evaluate the in vitro safety and technological characteristics of isolated beneficial canine probiotic strains.
- To identify suitable strains for a multi-strain probiotic formulation for dogs.
Main Methods:
- Screening of 100 canine isolates for inhibitory substance production using plate diffusion.
- In vitro safety assessment of 30 pre-selected strains, including antibiotic resistance and virulence factor analysis.
- Evaluation of 10 selected strains for tolerance to gastrointestinal conditions, heat, and solute concentration, plus inter-strain compatibility.
Main Results:
- Some strains exhibited inhibitory activity against common pathogens; 38% produced hydrogen peroxide (H₂O₂).
- Antibiotic resistance genes were found in only one strain; no gelatinase or lecithinase activity was detected.
- Tolerance to stress conditions varied by strain; Lactobacillus johnsonii 67, Lactiplantibacillus plantarum 74, Ligilactobacillus salivarius 84, and Pediococcus acidilactici 81 demonstrated compatibility.
Conclusions:
- Selected canine probiotic strains possess desirable in vitro safety and technological properties.
- Four compatible strains (L. johnsonii 67, L. plantarum 74, L. salivarius 84, P. acidilactici 81) are suitable for a multi-strain canine probiotic formulation.
- Further evaluation in vivo is warranted for the developed probiotic formula.
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