Related Experiment Video
Updated: Jan 9, 2026
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Development of a reliable and convenient lateral flow immunoassay for dihydromyricetin detection in Ampelopsis
Xueyan Cui1, Zhen Cao2, Hailong Yu3
1State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China; School of Bioengineering, Beijing Polytechnic University, No. 9 Liangshuihe 1st Street, Beijing 100176, China.
Abstract:
Dihydromyricetin, a flavonoid abundant in Ampelopsis grossedentata distributed across southern China, exhibits diverse pharmacological activities. In this study, a precise, sensitive, rapid, and reliable lateral flow immunoassay (LFIA) was developed for dihydromyricetin quantification in Ampelopsis grossedentata. A dihydromyricetin hapten was synthesized by introducing a carboxylic acid spacer via a four-carbon chain at the 3-position. A hybridoma cell line (2H3) secreting a highly specific monoclonal antibody (mAb) against dihydromyricetin was established. Dihydromyricetin demonstrated excellent stability in most organic solvents and acidic conditions. The LFIA exhibited a sensitivity (IC₅₀) of 33.61 ng/mL and a linear range of 4.54-281.83 ng/mL. The recovery rates of dihydromyricetin were in the range of 70.83-96.17 %. These findings indicate that LFIA, leveraging the high specificity of the dihydromyricetin mAb, offers a simple, rapid, and accurate method for dihydromyricetin analysis in Ampelopsis grossedentata.
