LPSN-API Assistant: A Windows standalone tool for automating the bulk collection and organization of type strain 16S...

The List of Prokaryotic Names with Standing in Nomenclature (LPSN) is a robust platform for prokaryotic taxonomists, providing the latest taxonomic information and 16S rRNA gene sequences. The type strain 16S rRNA gene sequence is a cornerstone in microbial taxonomy and an essential resource for researchers in the field of microbiology. However, manually collecting taxonomic information, downloading, and managing 16S rRNA gene sequences from LPSN can be time-consuming and laborious. The LPSN-API is a reliable tool for systematic microbiologists, yet for users with no experience in R or Python programming, readily applying the LPSN-API for taxonomic data collection and organization remains a challenge. To address this general challenge, we developed LPSN-API Assistant (version 1.0), a non-commercial Windows standalone tool with a user interface developed using Python to automate the collection of taxonomic information and bulk download of type strain 16S rRNA gene sequences of a given genus from the LPSN. LPSN-API Assistant streamlines the information collection and sequence downloading process, and provides functionalities for fast organizing and formatting sequence data of specified prokaryotic genera for phylogenetic analysis. Here, we present the design, implementation, and application of LPSN-API Assistant (https://gitee.com/rashidin-abdugheni/lpsn-api_-assistant_1.0), highlighting its applicability and potential to assist prokaryotic taxonomists.