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Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...

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IPEC-J2 as a cellular model for studying intestinal mucus.

Frédéric Dessauge1, Annie Vincent1, Christine Trefeu1

  • 1PEGASE, INRAE, Institut Agro, Saint Gilles, 35590, France.

Scientific Reports
|December 9, 2025
PubMed
Summary
This summary is machine-generated.

Porcine jejunal cells (IPEC-J2) can produce mucus, particularly under air-liquid interface (ALI) and agitated serum conditions. This finding establishes IPEC-J2 cells as a valuable in vitro model for studying intestinal mucus and nutrition.

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Area of Science:

  • Cell Biology
  • Gastroenterology
  • Epithelial Biology

Background:

  • Intestinal mucus is vital for protection and nutrient absorption.
  • The IPEC-J2 cell line is a model for intestinal barrier function.
  • IPEC-J2 mucin expression is inconsistently reported.

Purpose of the Study:

  • Investigate mucin expression in IPEC-J2 cells.
  • Determine optimal culture conditions for mucus production.
  • Validate IPEC-J2 as a model for mucus-nutrition interactions.

Main Methods:

  • Cultured IPEC-J2 cells under varying serum concentrations, agitation, and air-liquid interface (ALI).
  • Assessed mucus production using functional and structural methods.
  • Analyzed mucin (MUC2, MUC3, MUC13) and TFF3 expression via immunohistochemistry and flow cytometry.

Main Results:

  • Mucus production (MUC2, TFF3) significantly increased in ALI and 5% porcine serum with agitation (5PSAg) cultures.
  • Membrane mucins MUC3 and MUC13 were detected, with MUC13 localization differing from jejunal tissue.
  • Approximately 8% of IPEC-J2 cells expressed MUC2 in 5PSAg, similar to in vivo goblet cell levels.

Conclusions:

  • IPEC-J2 cells can differentiate into mucus-secreting cells under specific conditions (ALI, 5PSAg).
  • This cell line serves as a suitable in vitro model for studying mucus-food component interactions.
  • The findings support the use of IPEC-J2 cells in nutritional research.