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Related Experiment Video

Updated: Jan 9, 2026

Construction and Characterization of a Novel Vocal Fold Bioreactor
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Mouse Vocal Fold Permeability In Vivo: Effects of Novel Low-Tech Injury and Instillation Methods.

Renee E King1, Ella T Ward-Shaw1, Paul F Lambert1

  • 1McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, Wisconsin, USA.

The Laryngoscope
|December 10, 2025
PubMed
Summary

Simple low-tech methods effectively injure and instill mouse vocal folds (VFs). These techniques offer a promising alternative for studying laryngeal pathologies and developing new treatments.

Keywords:
animal modeldrug deliveryepithelial permeabilitylarynxvocal fold injury

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Area of Science:

  • Laryngology
  • Toxicology
  • Animal Models

Background:

  • Vocal fold (VF) injury and instillation are crucial for studying laryngeal pathologies in mouse models.
  • Current methods often require advanced visualization and prolonged anesthesia.
  • Developing simpler, low-tech approaches is essential for broader accessibility.

Purpose of the Study:

  • To evaluate the efficacy of intraperitoneal (IP) naphthalene (NAPH) and oropharyngeal aspiration (OA) of polidocanol (PDOC) for injuring and instilling mouse VFs.
  • To determine if chemical injury increases in vivo VF epithelial permeability.
  • To assess the feasibility of these methods for laryngeal research.

Main Methods:

  • Mice received varying doses of IP NAPH or OA of PDOC to induce VF injury.
  • Twenty-four hours post-injury, Evans blue (EB) dye was instilled via OA.
  • VF injury was assessed, and EB permeability was quantified using fluorescence imaging.
  • Immunostaining was performed for basal cells, tight junctions, and basement membrane.

Main Results:

  • High-dose NAPH (350 mg/kg) and high-volume 2% PDOC resulted in 100% VF injury.
  • EB dye binding to the VF lamina propria occurred in up to 100% of mice.
  • VF injury did not significantly alter epithelial permeability.
  • Injured VFs showed reduced basal cells and tight junctions, while the basement membrane remained intact.

Conclusions:

  • IP NAPH and OA are effective low-tech methods for consistent mouse VF injury and instillation.
  • The inherent high permeability of uninjured murine VF epithelium is notable.
  • These methods provide accessible tools for laryngeal research, including genetic engineering and toxicology studies.