Related Experiment Video
Updated: Jan 9, 2026
Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model
Published on: May 6, 2020
Severe knee osteoarthritis is associated with histological and ultrastructural changes in the semitendinosus tendon
Dlshad Qadir1,2, Khaled Meknas3,4, Sonja Eriksson Steigen5,6
1Department of Orthopedics NU Hospital Group Trollhattan Sweden.
Purpose:
This study aimed to assess degenerative changes in the semitendinosus tendon in patients with knee osteoarthritis (OA). The hypothesis was that individuals with severe OA would demonstrate more pronounced histological and ultrastructural tendon degeneration than those with mild-to-moderate OA.
Methods:
Tendon samples were obtained from 45 patients with knee OA. Twenty patients (median age: 53 [30-63] years) with mild-to-moderate OA underwent high tibial osteotomy (HTO), while 25 patients (median age: 71 [51-88] years) with severe OA underwent total knee arthroplasty (TKA). Semitendinosus tendon biopsies were collected at the time of surgery and examined histologically and ultrastructurally using light and electron microscopy.
Results:
The severe OA group exhibited a significantly larger mean fibril diameter than did the mild-to-moderate OA group. Histological analysis revealed significant differences across all components of the semiquantitative scoring system, except in cellularity, which remained similar between groups. The overall degenerative score was significantly higher in the severe OA group.
Conclusions:
Advanced knee OA is associated with significant histological and ultrastructural degeneration of the semitendinosus tendon, which could be interpreted as an adaptive response to chronic joint degeneration and biomechanical stress.
Level Of Evidence:
Level III.
