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Effect of Matrix Metalloproteinase Inhibitors on the Bonding Durability of Nanocomposite Resin to Caries-affected
Faeiza D Abdel-Salam1, Noha Sheta2, El-Sayed Gad Eid3
1Department of Dental Biomaterials, Faculty of Dentistry, Sirte University, Sirte, Libya, Orcid: https://orcid.org/0009-0001-7332-3033.
Aim:
This in vitro study evaluated the microtensile bond strength (µTBS) of two nanohybrid resin composites to caries-affected dentin (CAD) after pretreatment with different hesperidin (HPN) concentrations, before and after thermocycling.
Materials And Methods:
Eighty extracted human molars were assigned to two groups by composite type (Filtek Z250 XT, Grandio; n = 40 each) and subdivided into four pretreatment protocols (n = 10): control (no treatment), 0.5%, 1%, or 2% HPN. Half of the specimens in each subgroup were subjected to thermocycling. Microtensile bond strength was measured using a universal testing machine. Data were analyzed using two-way and three-way ANOVA followed by post hoc tests (α = 0.05).
Results:
Bond strength was significantly affected by HPN concentration and thermocycling. Non-aged Filtek and Grandio showed higher values (up to 39.90 ± 10.67 and 45.87 ± 13.37 MPa, respectively), while aging reduced strength in specific subgroups (Filtek no-treatment: p = 0.016; Grandio 0.5% HPN: p = 0.004; 2% HPN: p = 0.033). Overall, 0.5% HPN maintained better adhesive performance after aging, whereas higher concentrations showed diminished stability. Failure mode analysis supported these trends, with higher bond strength linked to cohesive/mixed failures.
Conclusion:
Pretreatment with 0.5% HPN significantly improved bond strength and durability to CAD, while higher concentrations reduced performance, especially after thermocycling.
Clinical Significance:
Applying 0.5% HPN as a dentin pretreatment may enhance the longevity of composite restorations in cases involving CAD. How to cite this article: Abdel-Salam FD, Sheta N, Eid EG. Effect of Matrix Metalloproteinase Inhibitors on the Bonding Durability of Nanocomposite Resin to Caries-affected Dentin: An In Vitro Study. J Contemp Dent Pract 2025;26(11):1096-1102.
