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Updated: Jan 9, 2026

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Buc Maintains Maternal RNA Stability and Embryogenesis in Zebrafish.

Ran Miao1, Yan Xie1, Qingqing Han1

  • 1Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.

Cells
|December 10, 2025
PubMed
Summary
This summary is machine-generated.

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Bucky ball (Buc) protein stabilizes maternal mRNA during zebrafish development. Buc safeguards mRNA integrity, ensuring proper embryonic development through the maternal-to-zygotic transition.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The maternal-to-zygotic transition (MZT) is crucial for early vertebrate embryogenesis.
  • Maternal mRNA clearance and zygotic genome activation are key events during MZT.
  • Germ plasm (GP) factors regulate maternal mRNA stability.

Purpose of the Study:

  • To investigate the role of Bucky ball (Buc), a zebrafish GP organizer, in maternal mRNA stability.
  • To determine if Buc protects maternal mRNAs from degradation during early embryogenesis.

Main Methods:

  • Overexpression of the buc gene in zebrafish embryos.
  • Generation of buc knockout zebrafish.
  • Analysis of maternal mRNA degradation rates.
  • Assessment of embryonic developmental phenotypes.
Keywords:
Bucky ballIgf2bp3embryo developmentmaternal mRNAzebrafish

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  • Investigation of downstream targets, including Igf2bp3 expression.

    • Main Results:

    • Overexpression of buc delayed maternal mRNA degradation and embryonic development.
    • buc knockout led to accelerated maternal mRNA degradation and severe developmental defects.
    • Buc regulates maternal mRNA stability via the expression of RNA-binding protein Igf2bp3.

    Conclusions:

    • Buc stabilizes maternal mRNAs in coordination with Igf2bp3 during the MZT.
    • Buc plays a critical role in maintaining maternal mRNA integrity for proper embryonic development.
    • The function of Buc extends beyond GP assembly to safeguarding maternal mRNA.