Nocardioides secundeburneus sp. nov. and Gordonia aurantiaca sp. nov., isolated from soil

Two strains, B21^T and C4-1^T, were obtained from soil near Sinpung Station, the Republic of Korea. The two aerobic, Gram-negative, rod-shaped bacterial strains were designated B21^T and C4-1^T. Phylogenetic tree analysis based on 16S rRNA and whole-genome sequences suggested that the strains B21^T and C4-1^T belonged to the genera Gordonia and Nocardioides, respectively. The draft genome of strain B21ᵀ consists of 4.99 Mb with a G+C content of 68.3 mol%, while that of strain C4-1ᵀ is 4.80 Mb with a 72.8 mol% G+C content. The cells of strain B21^T were non-motile and grew at a temperature of 15-50 °C (optimum, 40 °C), in the presence of 0-11.0% (w/v) sodium chloride (NaCl) (optimum, 0.0%) and at pH 5.0-12.0 (optimum, 8.0). The cells of strain C4-1^T were non-motile and grew at a temperature of 15-30 °C (optimum, 30 °C), in the presence of 0-8.0% (w/v) NaCl (optimum, 0%) and at pH 5.0-10.0 (optimum, 8.0). The novel strains B21^T and C4-1^T exhibited average nucleotide identity values of 73.9-87.2% and 75.0-81.4% and digital DNA-DNA hybridization values of 19.9-32.8% and 20.0-24.0%, with reference strains in the genera Gordonia and Nocardioides, respectively. We thus propose the names Gordonia aurantiaca (type strain B21^T=KACC 23739^T=TBRC 19009^T) and Nocardioides secundeburneus (type strain C4-1^T=KACC 23738^T=TBRC 19008^T).