CL-20 biodegradation and gene analyses of Sphingobium sp. CzL-01 isolated from activated sludge

The potential health and environmental risks posed by 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20), a high-energy-density compound, have spurred research into microbial degradation strategies. While several CL-20-degrading bacterial strains have been isolated, their degradation efficiency remains suboptimal, underscoring the need for enhanced biodegradation methods to address CL-20 contamination. In this study, morphological characterization identified a novel bacterial strain, designated CzL-01, which exhibited CL-20 biodegradation capability when the compound was provided as the sole nitrogen source. This investigation employs a multi-faceted approach including 16S rRNA gene sequencing for phylogenetic analysis, quantitative assessment of CL-20 degradation efficiency, and complete genome sequencing of the isolate. This work marks the first documented investigation of the Sphingobium genus's role in high-energy-density material catabolism. Further characterization of this strain is anticipated to elucidate the microbial metabolic pathways involved in CL-20 degradation, thereby facilitating the development of advanced bioremediation protocols for wastewater treatment applications.